RNA

Part:BBa_K1994031:Design

Designed by: Egheosa Ogbomo   Group: iGEM16_Warwick   (2016-10-14)


sgRNA with PAM Proximal complementary sequence and MS2 binding protein handle


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 12
    Illegal NheI site found at 35
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 54
    Illegal BsaI.rc site found at 42


Design Notes

sgRNA with PAM Proximal complementary sequence and MS2 binding protein handle


Source

sgRNA with PAM Proximal complementary sequence and MS2 binding protein handle

References